Evaluation of arbitrarily primed PCR for typing of Desulfovibrio desulfuricans strains
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چکیده
منابع مشابه
Multicenter evaluation of arbitrarily primed PCR for typing of Staphylococcus aureus strains.
Fifty-nine isolates of Staphylococcus aureus and a single strain of Staphylococcus intermedius were typed by arbitrarily primed PCR (AP-PCR). To study reproducibility and discriminatory abilities, AP-PCR was carried out in seven laboratories with a standardized amplification protocol, template DNA isolated in a single institution, and a common set of three primers with different resolving power...
متن کاملComparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.
Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. However, the comparative typing ability, reproducibility, discriminatory power, ...
متن کاملSerine biosynthesis in Desulfovibrio desulfuricans.
Cell-free extracts of Desulfovibrio desulfuricans possess enzymes which catalyze the synthesis of serine from 3-phosphoglycerate via the intermediates phosphohydroxypyruvate and phosphoserine.
متن کاملGenetic transfer in Desulfovibrio desulfuricans.
An apparently defective bacteriophage capable of mediating transduction has been identified in culture filtrates of Desulfovibrio desulfuricans (American Type Culture Collection 27774). Phage-mediated intraspecies transfer of antibiotic resistance markers occurs with a frequency of 10(-5) to 10(-6) per recipient cell. The vector contains linear fragments of double-stranded DNA of about 13.5 kil...
متن کاملIncreased efficiency of arbitrarily primed PCR by prolonged ramp times.
for functional analysis. Mutagenesis to the small promoter-only containing plasmid is preferred to that of the reporter gene construct, because amplification of a smaller plasmid is faster and more efficient and removes the slight possibility of introducing random mutations to the reporter gene. The transferred mutant region can be sequenced to confirm that no random mutations were introduced d...
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ژورنال
عنوان ژورنال: Microbiological Research
سال: 2003
ISSN: 0944-5013
DOI: 10.1078/0944-5013-00189